Classical conditioning (also respondent conditioning and Pavlovian conditioning) is a behavioral procedure in which a biologically potent physiological stimulus (e.g. food) is paired with a neutral stimulus (e.g. the sound of a musical triangle). The term classical conditioning also refers to the subject animal's learning from the pairing of a physiologic stimulus with a neutral stimulus, which elicits the required response (e.g. salivation) from the neutral stimulus rather than the physiological stimulus.

The Russian physiologist Ivan Pavlov studied classical conditioning with detailed experiments with dogs, and published the experimental results in 1897. In the study of digestion, Pavlov observed that the experimental dogs salivated when fed red meat. Pavlovian conditioning is distinct from operant conditioning (instrumental conditioning), through which the strength of a voluntary behavior is modified, either by reinforcement or by punishment, however, classical conditioning can affect operant conditioning; that classically conditioned stimuli can reinforce operant responses.

Classical conditioning is a basic behavioral mechanism, and its neural substrates are now beginning to be understood. Though it is sometimes hard to distinguish classical conditioning from other forms of associative learning (e.g. instrumental learning and human associative memory), a number of observations differentiate them, especially the contingencies whereby learning occurs.

Together with operant conditioning, classical conditioning became the foundation of behaviorism, a school of psychology which was dominant in the mid-20th century and is still an important influence on the practice of psychological therapy and the study of animal behavior. Classical conditioning has been applied in other areas as well. For example, it may affect the body's response to psychoactive drugs, the regulation of hunger, research on the neural basis of learning and memory, and in certain social phenomena such as the false consensus effect.